Traditional research microscopy tools are entirely adequate for conventional static analysis, however, the evaluation of more complex dynamic cellular processes require unconventional methods of acquiring, displaying, and rendering dynamic data. Autophagy occurs when some of a cells cytoplasm, referred as cargo, is enclosed by a membrane to form an autophagosome in order to be degraded and the products recycled. Autophagy is initiated to cope with stress, but we and others show that under non-stress conditions, specific cargos are targeted to the autophagosome.