Création d’un panel pharmacogénomique et élaboration d’une base de données translationnelle

À chaque année, des milliers d’individus souffrent ou décèdent suite à l’administration d’un médicament qui n’était pas approprié pour eux. Grâce aux récentes découvertes en génétique, il est maintenant possible de mieux prédire comment un individu va répondre à divers médicaments en analysant ses variations génétiques. Hors, un service de séquençage génétique n’est toujours pas disponible pour la population canadienne.

Investigating the effects of ?-adrenergic stimulation on IKs channel trafficking in cardiac myocytes using total internal reflection fluorescence (TIRF) microscopy

Cardiac arrhythmia is an electrical disturbance in the heart which can cause a variety of potentially life threatening conditions. Arrhythmias are due to malfunctions in the protein channels, which in turn alter the bioelectric currents signaling each heartbeat. A key channel responsible for terminating the electrical signal is the voltage gated potassium channel, IKs. During an adrenaline spike associated with the fight or flight response, there is an increase in IKs activity although the mechanism is not well understood.

An economic evaluation of sulfonylurea medication, hypoglycemic episodes and falls in older adults with type 2 diabetes

Drug use in older persons is a major public health concern. Even though therapeutic drugs are beneficial for patients’ health in terms of survival or quality of life, patients aged >65 years have a greater risk of developing drug-related complications. Such complications may be fatal because of the high frequency of both multiple pathologies and polypharmacy in these patients, who consume a major proportion of healthcare resources.

Potential of magnetic nanoparticle targeting with the help of cell permeable proteins

Magnetic drug targeting is a recent drug delivery method where the drug is bound to a carrier, the magnetic nanoparticles. Directed with a magnetic field towards the target tissue, e.g., tumours. the drug is released there to produce a therapeutic effect. In many cases, however, it is additionally necessary that the drug enters the target cells to be active.

Development of Lipid Nanoparticle Reagents for Functional Genomics in Difficult-to-Transfect Cells In Vitro

The objective of this project is to develop lipid nanoparticle (LNP) reagents for the delivery of nucleic acids to turn off, or turn on target genes in “hard-to-transfect” neurons and stem cells in vitro and in vivo. A recent report (BCC Research, April 2011) observed that "51% of researchers employ cell-based techniques to perform transfection routinely.

Design and synthesis of novel influenza M2 proton channel inhibitors with drug resistant antiviral activity

Seasonal influenza causes significant mortality and morbidity worldwide, and we are currently unprepared to mitigate a fatal pandemic outbreak like the 1918 Spanish Flu. A proven influenza antiviral target is the M2 viroporin, or viral ion channel. Amantadine, an M2 inhibitor was an effective antiviral for 20 years. However, current influenza antivirals are increasingly ineffective as viruses develop resistance. For example, the M2 mutation S31N is now present in >90% of influenza strains and confers resistance to adamantanes.

Regulation of T-type calcium channel activity by targeting channel trafficking – a novel approach for pain management Year two

Current therapies to manage pain either result in side effects or are insufficient and the associated medical costs and loss of work days come pose a tremendous socioeconomic burden. We recently showed that T-type channel activity is aberrantly regulated in inflammatory and neuropathic pain by the deubiquitinase USP5, and we have begun to explore this mechanism as a new therapeutic avenue based on interfering TAT peptides. We now plan to test our TATpeptides in diabetic neuropathy and inflammatory bowel pain.

Investigating the biophysics and structural basis for state dependent drug blockade of persistent/late sodium current (INa(P)) in the heart using photoactivatable crosslinking unnatural amino acidsYR2

Electrical activity in the heart is controlled by the concerted activity of many proteins called ion-channels that regulate the transfer of different ions across cell membranes. Recently, researchers in biomedical science have identified that a particular component of sodium carrying ion-channel activity (called the persistent or late sodium current also known as INa(P)) played a major role in controlling the electrical activity of the heart. More recent research suggests that this late sodium current may be involved in various cardiac diseases.

Radiochemistry for the Pharmacokinetic Evaluation of Novel Bone-Targeting Peptide Hormone Derivatives

This proposed research project is about development of new bone-targeting drug called PTHPEG- BP, this new compound will overcome shortages of current clinical peptide hormone PTH, and show better treatment efficacy and lower price then the latter; several new technologies will be used on research of this PTH, such as micro Positron Emission Tomography (PET/CT), and several of its characteristics will be identified such as structure, bioactivity, and metabolism inside body.

Regulation of T-type calcium channel activity by targeting channel trafficking – a novel approach for pain management

Current therapies to manage pain either result in side effects or are insufficient and the associated medical costs and loss of work days come pose a tremendous socioeconomic burden. We recently showed that T-type channel activity is aberrantly regulated in inflammatory and neuropathic pain by the deubiquitinase USP5, and we have begun to explore this mechanism as a new therapeutic avenue based on interfering TAT peptides. We now plan to test our TATpeptides in diabetic neuropathy and inflammatory bowel pain.

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