Development of Novel Methods for the Automated Extraction of Lipid-Soluble Compounds Year One

Current analytical methods for the extraction of lipid-soluble compounds (fatty acids, cholesterol, antibiotics, pesticides, vitamins) are costly and time-consuming. Recently, we have assessed a novel patent-pending “kit” method for extracting fatty acids and cholesterol from food and tissues with great success. We propose to continue testing this method for extraction of lipid-soluble vitamins, pesticides and antibiotics for the continued sale of kits to potential research laboratories.

Computer aided system based on Image processing technique for assessment ofspinal cord from MRI Year One

The spinal cord is one of the most important neurological structures in the body. Noninvasive image-based assessment of this structure is an increasingly demanding healthcare service. This project is a preliminary development stage of a computer-aided spinal-scan assessment software toolkit which aims at faciiitating the diagnosis of spinal cord problems. In parlicular, the proposed project is to design computational methods which automatically extract image fearures.

Isolation, characterization and culture maintenance of stem cells from human hair follicle mesenchyme Year Two

Stem cells and other pluripotent cells are under active investigation for the development of new therapeutic treatments to regenerate and repair diseased and damaged tissues. Published studies indicate that some mesenchymal cells derived from adult human hair follicles exhibit stem cell properties and can form different cells types if they are given specific biological signals without the need to genetically modify the cells.

Transcriptomic analysis of follicular somatic cells after vitamin supplementation using Vicomb® feed additive in bovine Year Two

In cattle, energy requirements after parturition increase exponentially, primarily, due to increase in milk production. Negative energy balance hampers reproductive efficiency, inflicting heavy economic losses to dairy industry. Poor reproduction is the most important cause of culling dairy cows. Feed supplementation with vitamins improves energy balance. Also, observations in humans indicate that folic acid and vitamin B12 improve oocyte quality and pregnancy establishment.

Development of Fast Longitudinal Cortical surface extraction, deformation, and Normalization (FALCON) for the measurement of cortical thickness over time from brain magnetic resonance images Year Two

The project involves development, validation, and application of new medical image analysis algorithm where cortical thickness is measured from serial brain magnetic resonance imaging (MRI) with improved efficiency and greater sensitivity. Cortical thickness is a relatively new metric that correlates with clinical and cognitive worsening in a variety of neurologic disorders such as multiple sclerosis and Alzheimer's disease.

Postnatal development in mice and rabbits: an histomorphological study

his study focuses on the postnatal histomorphology of developing organs in mice and rabbits and compares the key events between human and experimental species (mice and rabbits). It will provide an essential resource for nonclinical safety evaluations of pediatric drugs and will be helpful in preparing appropriate study designs and testing strategies. Neonatal and juvenile organs greatly differ morphologically and functionnally from adult animals.

Evaluating New Methods for Monitoring Cognitive Health Year Two

Copeman Healthcare Centre is engaged in a multi-year research and development program in the area of cognitive health. Cognitive health is a surrogate marker for brain health. All other technologies, such as biomarkers, electrophysiology, structural brain imaging, and functional brain imaging attempt to measure damage to the brain. Moreover, these technologies do not measure or quantify the healthy brain or its functioning; they are used to detect what appears to be wrong based on anatomical or physiological indicators.

Evaluating New Methods for Monitoring Cognitive Health

Copeman Healthcare Centre is engaged in a multi-year research and development program in the area of cognitive health. Cognitive health is a surrogate marker for brain health. All other technologies, such as biomarkers, electrophysiology, structural brain imaging, and functional brain imaging attempt to measure damage to the brain. Moreover, these technologies do not measure or quantify the healthy brain or its functioning; they are used to detect what appears to be wrong based on anatomical or physiological indicators.

Development of 3D culture reagents and scoring systems for assays of normal andmalignant epithelial stem cells Year Two

The overarching objective of this proposal is to develop and validate a new, robust and reproducible 3D culture system that supports the clonal growth of primary sources of normal and malignant human epithelial cells and that is suitable for commercialization by STEMCELL Technologies who will partner in its development. The work will systematically address 3 Specific Aims.

Phase I: Using simultaneous EEG-fMRI to study theaffective mechanisms underlying obsessivecompulsivedisorder

The aim of this project is to improve simultaneous EEG-fMRI techniques through the use of constrained principal component analysis (CPCA). The main problem with combining EEG measures and fMRI measures is that recording EEG in the MR scanner always injects noise into the data. CPCA partitions the variability of the data into systematic and error variance.

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