Overall aim of the project: To establish the protocol for oocyte cryopreservation which results in a high success rate of embryo development. We will first use mouse oocytes to test the effects of various compounds supplemented in the cryopreservation medium on subsequent oocyte competence. The mouse has been useful model for elucidating various aspects of oogenesis and reproductive development events including, global oocyte gene expression, intra-oocyte redox state, and critical events during meiotic initiation in the ovary, follicle formation and oocyte growth and maturation.
1) To capture and compare microRNA (MIR) signatures in mouse ovarian follicular fluid and spent media of cumulous cells-oocyte complexes (COCs) isolated from infertility mouse models to identify one or more unique MIR signatures that will distinguish competent oocytes from incompetent oocytes.
Peptide therapeutics are a novel approach to treat bacterial and viral infections, which have major advantages over small molecules. Peptide molecules have been used previously to treat HIV infections, and I have previously shown that peptide molecules targeting Chlamydia can prevent infection in vivo. Project Objectives: 1. To take previously characterized peptides, move them onto an HSA scaffold, and evaluate their expression in a yeast system. 2. To test the HSA scaffold therapeutic peptides in vitro against Chlamydia infection, RSV infection, and influenza infection. 3.
My objectives will be 1) to establish stable zebrafish lines expressing the human nuclear receptors not yet established by Indanio; 2) to de-orphanize at least three human nuclear receptors; 3) to lead initiatives in drug target identification and validation; and 4) to establish protocols for structural and pharmacokinetic analysis of potential drug targets. 1) Establishing the zebrafish lines will be accomplished using the Indanio-designed reporter system and standard molecular biology techniques. 2) The strategy to adopt orphaned nuclear receptors is a multistage project.
To date, the Shoichet laboratory has focused on a material which incorporates both a therapeutic and HAMC however, combining these elements complicates the commercialization. From the published literature we have a good indication that HAMC could be beneficial if delivered without an additional therapeutic. Two reports investigating delivery of HAMC alone have shown beneficial effects (a,b). These two reports used intrathecal delivery of HAMC to the spinal cord and observed dural healing in addition to reduced inflammation.
The primary project objective of this proposal is to evaluate company’s absorbable polymeric (PCL/PLLA) performance as the heat flowable bone repair adhesive materials. The expected overall project outcome is a unified surgical delivery tool and implantable adhesive that provides the customers/users (surgeons/patients/hospitals) of this device a surgical repair product. Materials and Methodology: Material Description: The UMass patent covers a blend of two well-known absorbable materials Polycaprolactone (PCL) and poly(L-lactide) (PLLA).
Cannabinoids are synthesized and accumulate in Cannabis plant hairs called glandular trichomes, which are particularly abundant on female flower buds. Cannabinoid content and composition vary dramatically among Cannabis strains, with two general groupings distinguished: marijuana strains, defined by high THC and low CBD levels, and hemp strains containing low THC and high CBD (or low total cannabinoid content).
The overarching objective of this proposal is to develop and validate a new, robust and reproducible 3D culture system that supports the clonal growth of primary sources of normal and malignant human epithelial cells and that is suitable for commercialization by STEMCELL Technologies who will partner in its development. The work will systematically address 3 Specific Aims.
Thyroid cancer is the most frequent endocrine cancer. Over 90% of thyroid cancers are of the follicular or papillary type termed differentiated thyroid carcinoma (DTC). Thyroglobulin (Tg) serum measurement is an integral part of the follow up and management of DTC patients. Serum Tg levels are conventionally measured using either an immunometric assay (IMA) or radioimmunometric assay (RIA). Both methods rely on the use of labeled exogenous antibodies.
Sleep apnea is a common respiratory disorder among adult population; it is more probable among men and patients with fluid retaining conditions such as heart failure (HF) where it increases the mortality risk. Therefore, it is important to develop new treatments for sleep apnea. We have proposed that fluid which is accumulated in the legs during day moves towards the head when lying down to sleep and accumulates in the neck or lung; which could be a major contributor to the development of sleep apnea. Therefore, we aim to determine whether: