Characterizing intracellular interactions of carcinoembryonic-antigen-related cell adhesion molecule 1-4L using high resolution microscopy

Preliminary work has shown that adding excess CEACAM1-specific antibody, 26H7, to hela cells expressing carcinoembryonic-antigen-related cell-adhesion moleule (CEACAM) 1-4L decreases CEACAM1-4L in the plasma membrane and shifts their surface expression from a monomer-oligomer mix to mainly monomers. A super resolution microscope will be used to co-localize CEACAM1-4L with 26H7 in order to determine the optimal antibody dose, and how different doses affect CEACAM1-4L spatial distribution and surface expression. A microscope equipped for 3D imaging of live thick samples will be modified to be able to determine the the monomer-oligomer mix of CEACAM1-4L upon addition of 26H7, in 3D cell structures. Intracellular vesicles leaving the plasma membrane will be tracked and quantified. It is predicted that CEACAM1-4L dimers are being internalized, so I expect to see mainly monomers at the membrane, and an increase in CEACAM1-4L dimer containing vesicles leaving the membrane.

Faculty Supervisor:

Christopher Yip

Student:

Partner:

Université Paris Descartes

Discipline:

Engineering

Sector:

Education

University:

University of Toronto

Program: