Creating a genome-wide CRISPR interference functional genomic library in Candida albicans to study fungal gastrointestinal colonization

Candida albicans is an important human fungal pathogen and commensal member of the microbiota. It colonizes the skin, gut, and reproductive tract of most healthy individuals without complications – a state known as commensalism. However under conditions that weaken the immune system, antibiotic use, and damage to the lining of the gut, C. albicans can cause life-threatening systemic infections. These infections often originate from the gut whereby C. albicans translocates into the bloodstream and causes damage to its host, as a pathogen. Recent research has focused on understanding C. albicans as a pathogen and less so on its commensalism. This proposed research project aims to understand how different genetic factors may play a role in C. albicans colonization of the gut, using a gene editing tool, called CRISPR interference (CRISPRi). We will also use a murine model of gastrointestinal colonization developed and optimized by the host laboratory (Dr. Ilse Jacobsen) to screen >35,000 mutant strains and determine which genes in the C. albicans genome are important for colonization and survival in the gut. This research holds potential for understanding C. albicans commensal lifestyle, inform us of possible strategies to prevent infection, and deepen our understanding of C. albicans biology altogether.

Faculty Supervisor:

Rebecca Shapiro

Student:

Partner:

Leibniz Institute for Natural Product Research and Infection Biology

Discipline:

Life Sciences

Sector:

Education

University:

University of Guelph

Program: