Generation of S9 from fish cell lines as an alternative to live animals

Live animal testing is currently the standard approach for assessing the risk chemicals may pose to wildlife. The costs and ethical concerns associated with live animal testing, however, constitute a significant challenge to regulators and industry in fulfilling their testing mandates to characterize the risks of environmental contaminants. To reduce these challenges, improved exposure assessments that assess chemical risk at target tissues are needed. In vitro assays that characterize biotransformation of chemicals, in combination with in silico (i.e. toxicokinetic modelling) approaches are a proposed alternative to reduce the number of animals used in chemical testing. The current in vitro assays however, use hepatocytes or the S9 fraction of the liver obtained from a live animal. To eliminate the need for live animals to obtain the tissue, generation of S9 from permanent cultures of liver cells is proposed.TO BE CONT’D

Faculty Supervisor:

Markus Brinkmann;Markus Hecker

Student:

Partner:

Rheinisch-Westfälische Technische Hochschule Aachen

Discipline:

Life Sciences

Sector:

Education

University:

University of Saskatchewan

Program: