Identifying Markers of Developmental Competence Using Pluripotent Stem Cell- Derived Blastoids

Currently, embryo implantation success rates in in vitro fertilization (IVF) are low, partly due to subjective and invasive tests. An assay that can quantify embryo potential while preserving its integrity is necessary. Small RNA sequences, microRNAs (miRNAs), are promising, quantifiable, and non-intrusive markers of developmental potential in embryos. Due to ethical concerns with human embryos in research, an alternative model is needed. Recent findings demonstrate the ability of human embryonic stem cells to self-organize into pre-implantation analogues known as blastoids, but it is unknown if blastoids also release development-specific miRNA. We aim to demonstrate blastoids as a model for human embryo potential using miRNAs and ultimately develop an accurate and efficient embryo selection assay. This will improve IVF accessibility, affordability, success rates, and patient satisfaction while reducing operational expenses. Implementing this advanced technology will give the fertility clinic a competitive advantage, enhancing its long-term reputation for trust and credibility.

Faculty Supervisor:

Dean Betts

Student:

Partner:

London Health Sciences Centre

Discipline:

Life Sciences

Sector:

Health and Related Sciences & Technology

University:

The University of Western Ontario

Program: