Sensitive In situ Chromatic Biosensors for Detection of ESKAPE pathogens: Boosting Sensitivity, Preventing False Positive, Design Freeze

The developed Nanobiosensor is based on electrospun nanofibres with a core-shell structure. The core of fibres is based on polyurethane, and the shell is based on polyurethane, polyvinylpyrrolidone, hemicyanine dye, surfactant and acid. The HCy dye (colorimetric probe) changes colour when bacteria secrete lipase. The lipase cleaves the ester bond of the HCy, and the probe changes its colour due to intramolecular charge transfer. The incorporated surfactant increases lipase activity by providing a water-oil surface. When more lipase is activated, it can be possible for the HCy dye to change its colour in a lower concentration. Therefore, the sensitivity of the Nanobiosensor is boosted, and bacteria presence can be detected before local infection. The incorporated acid not only can help with faster wound healing but also prevents any false colour change that may happen due to changes in wound pH or the microenvironment of the wound. This innovation helps to monitor the wound in real-time with in situ detection and easy readout. The colour-changing can be recognized with the untrained and naked eyes and is compatible with different wound dressings.

Faculty Supervisor:

Song Liu

Student:

Partner:

ParaNano Wound Care, LLC

Discipline:

Engineering

Sector:

Manufacturing

University:

University of Manitoba

Program: