Small Molecule Ice Recrystallization Inhibitors as Cryo-additives for Red Blood Cell Cryopreservation

The transfusion of red blood cells (RBCs) is a lifesaving procedure for many patients. While the Canadian blood supply is safe and consistently meets demand, the primary method for the storage of RBCs prior to transfusion is refrigeration at 4°C for a maximum of 42 days, a process which can lead to inefficiencies and discards. Cryopreservation is a desirable method for the long-term storage of RBCs, providing access to a large quantity of RBCs required in emergency transfusions or for patients with unique transfusion requirements. However, current cryopreservation methods employ high concentrations of glycerol which must be removed prior to transfusion. Not only is the deglycerolization process costly and time-consuming, deglycerolized RBC units have a limited shelf-life of 24 hours. The proposed project will investigate the use of ice recrystallization inhibitors to optimize the cryopreservation of RBCs and improve access to frozen RBCs for patients in need.

Faculty Supervisor:

Robert Ben

Student:

Partner:

Canadian Blood Services (ON)

Discipline:

Life Sciences

Sector:

Biotechnology; Health and Related Sciences & Technology

University:

University of Ottawa

Program: