The application of the standardized RTgill-W1 cell line test in assessing the toxicity of pulp and paper effluents

Due to the lack of available standardized and scientifically defensible alternative test methods, millions of fish are used annually to evaluate the toxicity of effluent samples for regulatory purposes. Despite global efforts to reduce animal testing, few methods have been able to bridge the gap from a research technique to regulatory acceptance in Canada. One method that holds much promise uses a permanently cultured rainbow trout gill cell line to determine toxicity. This research aims to validate this test method for assessing the complex effluent from pulp and paper mills and has the potential to advance the use of alternative methods in regulatory toxicology. By reducing the use of vertebrates for toxicity testing, providing faster results, and requiring smaller volumes of effluent for testing, these methods will benefit pulp and paper clients, the Canadian environment and animal welfare.

Faculty Supervisor:

Joanna Wilson;Lisa Taylor

Student:

Partner:

Nautilus Environmental Company Inc.

Discipline:

Life Sciences

Sector:

Agriculture; Professional, scientific and technical services

University:

McMaster University

Program: