Antibiofilm molecules active agains staphylococci / Molécules antibiofilm actives contre les staphylocoques (Nouveau)
Bacteria within biofilms can withstand the host immune responses, and they are significantly more tolerant to antibiotics and disinfectants. Our laboratory found that some coagulase-negative staphyloccal (CNS) isolates can efficiently block biofilm formation by other CNS species or Staphylococcus aureus. Hypothesis: Some CNS isolates produce antibiofilm molecules that can lead to the development of new drugs and/or new strategies to control staphylococcal infection through biofilm dispersion or prevention. Objectives : (i) to determine the mechanism underlying the antibiofilm activity of CNS and (ii) the spectrum of activity on different bacterial species from our collection. Methodology : Biofilm production by bacterial isolates will be measured after growth in microtiter plates and quantification of the biofilm layer by using a standard staining procedure. We will investigate the ability of CNS to secrete enzymes with protease, lipase and/or nuclease activities using standard microbiological and biochemical methods. We will investigate if CNS can produce antibacterial compounds, known as bacteriocins. The spectrum of antibiofilm activity will be determined using a collection of CNS and S. aureus isolates, and of other bovine mastitis pathogens such as Steptococcus uberis, Strep. dysgalactiae, and E. coli. These antibiofilm molecules, used alone or in combination with an antibacterial agent, may represent a novel strategy to control bacterial infections.
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Les biofilms bactériens représentent un problème important en santé animale et en santé humaine à cause de leur résistance accrue aux antibiotiques et aux désinfectants. Notre laboratoire a mis en évidence que certaines souches de staphylocoque à coagulase négative (SCN) pouvaient bloquer d’une manière très efficace la formation de biofilms par d’autres staphylocoques dont S. aureus. Nous émettons l’hypothèse que ces souches de SCN produisent des molécules antibiofilm ce qui pourrait mener au développement de nouvelles drogues ou de nouvelles stratégies pour contrôler la formation de biofilm. Nos objectifs sont: 1)
