Development of a novel IPM strategy for brown marmorated stink bugs (Halyomorpha halys) using RNA interference (RNAi) technology – Year two

Brown marmorated stink bug (BMSB) (Halyomorpha halys (Stål)) is an invasive pest with a large host range that includes many economically important fruits, vegetables, and row crops. Native to Asia, BMSB was first detected in North America in 1998 and since has become established in British Columbia, Quebec, and Ontario; and 44 American states. In order to develop efficient BMSB focused integrated pest management program (IPM), it is critical that novel alternative control tactics are investigated primarily because there are no effective insecticides available. RNA interference (RNAi) is a form of genetic control that has shown promise as a management tactic for BMSB. In this context, we aim to investigate RNAi for BMSB management. We will use genetic and proteomic tools to design new dsRNA (n = 10) in order to affect all BMSB life stages. We will test the effects of dsRNA, first independently then in multiple combinations, on BMSB populations raised in the laboratory. The most efficient combinations (n = 3) will finally be tested in the field to assess their efficiency under natural conditions. Our project will be the first step in the development and potential commercialization of a control of BMSB, efficient in the long term.

Faculty Supervisor:

Cynthia Scott-Dupree

Student:

Alexandra SEBASTIEN

Partner:

Bayer CropScience Canada

Discipline:

Environmental sciences

Sector:

Life sciences

University:

University of Guelph