Genetic mapping and characterization of loci controlling resistance to powdery mildew (Golovinomyces cichoracearum) in Cannabis sativa

High quality Cannabis sativa varieties are needed in Canada since the legalization of recreational Cannabis in fall of 2018. Unfortunately, powdery mildew, caused by the fungi Golovinomyces cichoracearum is a major disease of cultivated cannabis. We propose using high-throughput genotyping tools to map new resistance genes to this pathogen. Our approach will include genome wide association (GWA) mapping and map-based cloning strategies, involving phenotyping hundreds of individuals at reproductive maturity and through an in vitro approach. The candidate genes will be characterized for their positive selection patterns, and will be functionally validated using the Arabidopsis thaliana model system. The protein subcellular localization of the candidate genes will be observed in tobacco leaves thanks to a transient expression experiment. This project will help Aurora to create sustainable resistances to powdery mildew in high-quality varieties and will help Pauline to develop her career as a plant molecular geneticist.

Faculty Supervisor:

Loren Rieseberg

Student:

Pauline Duriez

Partner:

Aurora Cannabis

Discipline:

Other

Sector:

Professional, scientific and technical services

University:

University of British Columbia