Identifying Dominant Anaerobic Microorganismsfor Degradation of Benzene

Contamination of soils and groundwater with gasoline often results in the development of anaerobic
zones. Benzene is particularly persistent under anaerobic conditions even in the enhanced anaerobic
biodegradation (EAB) process. Degradation of benzene has been demonstrated under nitratereducing
condition, and sulphate-reducing condition. However, information of dominant indigenous
species within the microorganisms for degrading benzene is still limited. In order to identify the target
bacteria, a novel approach is required, which requires less biomass and provides direct evidence for
benzene incorporation. Enrichment of benzene-degrading microorganisms with [13C] benzene and
nutrients implementation is a prerequisite for target bacteria identification. Dominant bacteria identities
will be revealed by pyrosequencing. Benzene incorporation by the isolated bacteria will be
demonstrated by SR-FTIR spectromicroscopy analysis of [13C] benzene. In this manner it is believed
to provide direct evidence for benzene incorporation by dominant indigenous bacteria, and furnish
useful information about their structure, function, and dynamics….

Faculty Supervisor:

Jian Peng

Student:

Partner:

Stantec Consulting (Saskatoon, SK)

Discipline:

Engineering

Sector:

Professional, scientific and technical services

University:

University of Saskatchewan

Program: