Formulation and Toxicology of Bone-Targeting Nanoparticles

Bones undergo a renewal process by replenishing calcium mineral through a cellular process known as remodeling. Usually, it happens in a balanced manner, but in many bone diseases, the remodeling process is increased with regional bone metabolism. Bisphosphonate (BP) drugs selectivity and strongly bind to bone mineral and become incorporated at active sites of bone turnover in an increased amount.

Optimization of conformational epitopes against the NTS1 and P2Y6 receptors

The proposed project, performed with the company Immune Biosolutions, aims to develop superior antibodies for biological targets of interest for the treatment of colorectal cancer. Specifically, these targets are a class of proteins expressed on cell surface, which are notoriously difficult for the development of antibodies. The method involves making molecules look like the biological target, in order to elicit antibody responses which are more specific to the targets.

Development of novel biochemical and biophysical assays for the identification of inhibitors of Hypoxia Inducible Factor-2

Cancer cells are known for their unique capacity to survive and grow in a low oxygen tension environment in the middle of a poorly vascularized solid tumor. This adaptation, which is central to the tumorigenesis process, is mediated by precise cellular mechanisms allowing the regulation of gene expression. Thus, the development of small molecules to modulate the activity of transcription factors is of great therapeutic interest. In order to develop such molecules, we plan to finance, with the help of IntelliSyn Pharma and Mitacs, one M.Sc. student.

Molecular mechanisms of synapse remodelling

In the CNS the majority of excitatory synapses are formed onto specialized structures known as dendritic spines which can undergo shape changes in the time frame of minutes to days. This inherent structural plasticity of dendritic spines even lasts into adulthood. It is still unclear why certain spines are more likely to be maintained, to provide a physical substrate for long-term information storage, while others are more structurally plastic. We have recently discovered a novel process by which mature dendritic spines can be maintained.

Role of caspase-7 in colorectal cancer

Many diseases, including cancer, afflict the intestines. This is in large part due to the continuous renewal of epithelial cells, and the exposure to toxins from the diet and to infectious agents. Inflammation also contributes to the genesis of cancer, as illustrated by the link between chronic and ulcerative colitis and colorectal cancer (CRC). Caspases, a family of cysteinyl peptidases, play a crucial role in both inflammation and cancer avoidance via cytokine productions and apoptosis (cell death), respectively.

Rational design of opioid analgesics for treatment of chronic pain

Opioids are the most efficacious analgesics known, and elective in the treatment of acute severe pain. In contrast, their use in the management of chronic pain syndromes remains limited, requiring a compromise between preserving analgesic efficacy and controlling side effects such as respiratory depression, somnolence, nausea, constipation as well as abuse, dependence and analgesic tolerance.

Apelinergic system as a promising target for myocardialdysfunction and shock

Cardiovascular disease and sepsis are two of the biggest public health problems in North America. Increasing evidence further suggests they are linked. Recent studies in human healthy volunteers and in chronic heart failure patients have highlighted the apelin system as a potential target for drug development. We therefore propose to fully validate pre-clinically the potential of apelin as a new target for cardiovascular disease and septic shock.

Pharmacological and phytochemical characterization of immunomodulatory polysaccharides from American ginseng

Ginseng is a medicinal plant with a long history of traditional use. The medicinal properties of ginseng root have been attributed to two main classes of chemical compounds: ginsenosides and polysaccharides. While much is known about ginsenoside pharmacology, less is known about polysaccharide pharmacology. One of the most important properties of ginseng root polysaccharides is their ability to stimulate the immune system.

Transdermal delivery of peptide hormones to treat tibial bone stress fractures using DiffusiMax liposomal cream base

The goal of this Mitacs research proposal study is to develop and apply experimental methodology for transdermal delivery of bone-targeting peptide hormone drugs. We aim to deliver these compounds using an appropriate hydrophilic-hydrophobic Diffusimax liposomal drug delivery cream base to anatomically superficial bone, at the sites of stress fractures (also known as “shin-splints”) in order to trigger bone repair, reduce pain burden, and to minimize possible side effects if those drugs were used systemically.

Production of bioactive peptides through high ydrostatic pressure assisted enzymatic digestion of egg white

Egg yolk contains IgY antibodies that target various infectious disease and food allergens. IGY Inc is a company focused on utilizing chicken immune system for antibody production. IgY antibody contains 0.64% of total wet egg yolk, after cracking each egg IGY Inc generates 60-70g of liquid egg white which has a low commercial value. Egg white is a rich source of dietary protein and also known for its biological activities. Therefore the primary objective of this study is to develop a pragmatic technique to hydrolyze egg white to produce peptides with biological activity.