Development of standard sample processing procedure for multi-omics analysis of small intestinal samples collected by an ingestible capsule and understanding of relationship between gut microbiota and IgA production

Recent studies have shown that the gut microbiome works as an organ of the human body which produces bioactive molecules and metabolites. Since the gut microbiome is in close interaction with the human internal environment and changes in the compositions of gut microbiota can impact host physiology through many pathways. Precise determination of gut microbiota compositions depends on the ideal sampling methods which are non-invasive, has little cross-contamination, and collect samples at different sites. The current investigational swallowable SIMBA capsule by Nimble Science can provide a non-invasive, inexpensive, and convenient sampling method to collect small intestine microbiota. The aim of this study is to optimize and evaluate the efficiency of SIMBA sampling capsule using optimization of sample processing procedures and the determination of microbial diversity followed by metabolomic profiling of samples from healthy controls and patients compared with samples obtained by endoscopy and feces.

Faculty Supervisor:

Kathy McCoy

Student:

Partner:

Nimble Science Ltd.

Discipline:

Life Sciences

Sector:

Manufacturing; Professional, scientific and technical services

University:

University of Calgary

Program: