Human microproteins as host dependency factors of Zika virus

Zika virus is an important mosquito-transmitted human pathogen endemic to tropical and subtropical parts of the world and was responsible for the pandemic in South America in 2016. In pregnant women, the virus can cross the placenta and infect the brain of developing fetus leading to congenital abnormalities and life-long sequelae in newborn babies. Currently there are no vaccines or drug available against Zika virus. Viruses depend extensively on host proteins during infection. Hence, host proteins are attractive targets to develop antivirals. Recently, several thousand novel small proteins termed microproteins encoded by human genome were discovered. However, their role in virus infections is not yet explored. This project will explore the role of microproteins in Zika virus life cycle using a CRISPR/Cas9 based genetic screen which systematically deplete microproteins from cells and study their effect on virus replication. The microproteins showing significant effect on virus replication will be further screened against a library of natural compounds to identify small molecules that can target microproteins essential for virus replication. With this project, we expect to explore the currently unknown role of microproteins in Zika virus infection and initiate a drug development program targeting host microproteins.

Faculty Supervisor:

Anil Kumar Victoria Ansalem

Student:

Partner:

Applied Pharmaceutical Innovation

Discipline:

Life Sciences

Sector:

Professional, scientific and technical services; Retail trade

University:

University of Saskatchewan

Program: