Production of in vitro and in vivo bison embryos derived from sexed semen

In mammals, the sperm determines the sex of the resulting offspring. Semen sexing is a process whereby sperm are sorted into Y- (male) or X-chromosome (female) bearing gametes. Sexed semen may be used for artificial insemination or in vitro embryo production to create offspring of a desired sex. In a zoo setting, fewer males are […]

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Understanding the Role of Ex Situ Conservation to Enhance Public Understanding, Awareness, and Support for Biodiversity

The purpose of this research is to use an evidence-based approach to further the Toronto Zoo’s social science and education programs, in order to support in situ conservation and biodiversity in relation to Canadian goals and the Aichi Targets. Using a collaborative approach this research will result in the co-production of knowledge, where the researcher […]

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Technological advancements for data collection in animal research

The proposed research project is to program artificial intelligence, as produced by technology company EAIGLE, to monitor animals’ behaviour at the Toronto Zoo. The program will be capable of distinguishing where animals are in their enclosures, between individual animals, and which behaviours they are producing under different contexts. This technology will allow zoos, conservation areas, […]

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Cellular factors influencing the developmental potential of pure and hybrid bison

Assisted reproductive technologies can provide unique alternatives for wildlife managers interested in preserving genetically valuable individuals. The Canadian wood bison, currently listed as threatened, will benefit greatly from the use of in vitro fertilization and embryo banking as a method for producing genetically healthy, disease‐free herds. However, minimal or non‐existent knowledge of the fundamental aspects […]

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Influence of nuclear cytoplasmic interactions on developmental competence of bison

Somatic cell nuclear transfer (SCNT) is a unique technology that allows the preservation of the entire genome from an individual, thereby avoiding dilution of valuable alleles. This is an important criterion in endangered species preservation. Our interest in the Canadian wood bison, a threatened species, has brought us to consider the application of SCNT as […]

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Somatic cell reprogramming as a tool for the conservation of genetic resources from endangered species

Conservation of endangered species has become increasingly urgent. This is evident given the rate of species extinction has increased by 100 to 1000-fold, and global biodiversity has decreased. With current climatic changes, these concerns will only grow. Assisted reproductive technologies can play a vital role in endangered species conservation. Many approaches are currently being utilized […]

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Optimization of wood bison interspecific somatic cell nuclear transfer and embryo culture for species conservation

Interspecies somatic cell nuclear transfer (iSCNT) is a promising technique whereby a bison donor cell is transplanted into a domestic cattle oocyte. Different fibroblast donor cells have been demonstrated to have different embryonic outcomes. Problems with SCNT embryos have been also reported after in vitro culture. Toronto Zoo’s efforts have focused on wood bison iSCNT, […]

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