Intrafibrillar vs surface mineralization in bovine flexor tendon-derived collagen fibrils – an in vitro model of calcific tendinopathy of the human Achilles.
Calcific tendinopathy (CT) is a staged, reactive process where pre-calcific changes are followed by a calcific phase (formative, resting, resorptive) and by post-calcific remodelling in which hydroxyapatite-like deposits form and quite often resorb spontaneously. CT is most prevalent in the neck, foot and hip and within the foot. The Achilles is a well-documented site of CT, yet in all cases clinical imaging cannot resolve where mineral reside relative to collagen fibrils (intrafibrillar vs surface/extrafibrillar) which is essential to understand the impact of CT on tendons’ mechanical properties. To better understand mineral deposition in CT, we propose to develop an in vitro model that interrogates a formative-phase-like, acellular window at 37 °C using tendon-derived collagen fibrils from bovine SDF to determine mineral location and its fibrillar-scale nanomechanical signature under controlled conditions. In addition, we will take advantage of a recently published method to locally destabilize collagen molecular packing within single fibrils to test whether mechanically damage is required to nucleate the formative phase in CT.
View Full Project DescriptionLaurent Kreplak
Warsaw University of Technology
Life Sciences
Biotechnology; Health and Related Sciences & Technology; Biomanufacturing
Dalhousie University
Globalink Research Award